Fig. 2
SYD regulated the gene expression in the colon from DSS-induced mice. A PCoA based on all gene expression profile (Adonis: R2 = 0.396, P = 0.000). B Heatmap revealed the differences of gene expression (top 15 up-regulated DEGs and top 15 down-regulated DEGs by SYD). C Core PANTHER pathways that played important roles in SYD treatment. D The expressions of genes related to 5-HT metabolism in mRNA sequencing. E IHC of the colonic tissue with 5-HT antibody. Scale bars, 20 × , 100 μm. F The 5-HT concentrations in the faeces. G The expressions of genes related to 5-HT metabolism in the colonic tissue and the relative abundance of genes about 5-HT synthesis in the faeces. H The expressions of proteins related to 5-HT metabolism in the colon that confirmed in WB. Compared with NC, *P ≤ 0.05, **P ≤ 0.01; compared with M, #P ≤ 0.05, ##P ≤ 0.01