Table 2 Application examples of high content screening techniques
Method | Target | Drug library/Drug | Total drugs | Screening quantity | Screening drugs | Disease | Refs. |
|---|---|---|---|---|---|---|---|
HCS | Nitroreductase and the red fluorescent protein mCherry | Drug/compound library | 138 | 7 | Belinostat | Focal segmental glomerular sclerosis | [170] |
Epithelial mesenchymal transition | MedChemExpress (MCE) compound library | 306 | 5 | Camptothecin, dimethyl curcumin, artesunate et al | Fibrotic diseases and cancer | [171] | |
Transcriptional repressor Snail | Crude extract from the leaves of X. vielana | – | 24 | Compound 12 | Osteosarcoma | [172] | |
Schistosoma mansoni thioredoxin glutathione reductase (SmTGR) | Compound library | 29 | 2 | 2-[2-(3-methyl-4-nitro-5-isoxazolyl)vinyl]pyridine and 2-(benzylsulfonyl)−1,3-benzothiazole | Schistosomiasis | [173] | |
α-SMA, E-cadherin | Chinese herbal medicine Tong-Mai-Yang-Xin-Wan (TMYX) | 22 | 5 | Glycyrrhizic acid, glyasperin A, and licorisoflavan A | Chronic kidney disease | [174] | |
DNA, F-actin, and microtubules | FDA-approved drugs | 55 | 5 | Combination vinblastine/ispinesib | Triple-negative breast cancer | [175] | |
B7-H3 | FDA-approved drugs | 1114 | 55 | Ingenol-3-angelate | Osteosarcoma | [176] | |
123 protein targets | Target-selective inhibitor library | 600 | 25 | – | Osteosarcoma | [177] | |
Green fluorescent protein | targeted anti-cancer therapy drug library | 99 | 30 | Imatinib | Triple-negative breast cancer | [178] | |
Cardiac function | medicinal herbs-derived natural compounds library | 347 | 23 | Cyanidin chloride | Cardiomyopathy | [179] | |
Col-12 | Self-built library | 614 | 3 | Danshensu, lawsone, and sanguinarine | Collagen-related diseases | [180] | |
Intestinal neutrophil accumulation and ROS level | Peach Blossom Decoction, Pulsatilla Decoction, and Gegen Qinlian Decoction | 74 | 6 | Palmatine, daidzin, oroxyloside et al | Inflammatory bowel diseases | [181] | |
Sirt3 | Tongmaiyangxin pills | 21 | 5 | licoisoflavone A | Cardiac hypertrophy | [182] | |
Macrophage | Xuanfeibaidu Formula | 104 | 6 | Polydatin, isoliquiritin, acteoside et al | COVID-19 | [183] | |
α-SMA | A standard TCM compound library | 344 | 16 | Ligustrazine, glycyrrhizic acid, astragaloside iv et al | Chronic kidney disease | [184] | |
YFP-CL1 | Self-established TCM library | 89 | 3 | Salvianolic acid A, salvianolic acid B, and ellagic acid | Alzheimer's disease | [185] |