Fig. 6

Inhibition of SIRT1 expression blocks the beneficial effects of Cga on PA-induced liver injury. A Serum ALT activity. B Serum AST activity. C Serum TBA amount. D Representative images of H&E-stained liver sections (scale bars, 50 μm). E The mRNA levels of Fxr and its associated genes (Shp, Bsep, Mrp2, Cyp7a1, Cyp8b1) were evaluated by qRT-PCR (n = 6). F The expression of the proteins FXR, SHP, BSEP, MRP2, CYP7A1, CYP8B1 were measured by WB in mouse livers. Bar graphs show summary data (n = 3). Data are shown as the means ± SEM and analyzed by Student's t test or one-way ANOVA. ^**P < 0.01, ^*** P < 0.001 vs. TA; ^&&P < 0.01, ^&&&P < 0.001 vs. TA-Hyp