Fig. 7

SSS treatment alleviated the worsening permeability of OGD insulted bEnd.3 cells barrier due to delayed tPA administration by VIP/VIPR1 pathway. A Schematic diagram of the co-culture of bEnd.3 cells and N2a. B tPA administration increased the permeability of OGD insulted bEnd.3 cells barrier. C SSS-containing serum treatment maintained the integrity of OGD insulted bEnd.3 cells barrier after tPA administration. D VIP knockdown in N2a cells verified by immunofluorescence. E VIPR1 knockdown in bEnd.3 cells verified by western blotting. F Quantification analysis of expressions of VIPR1 in bEnd.3 cells infected with VIPR1 siRNAs. G Knockdown of VIPR1 in bEnd.3 cells attenuated the protective effects of SSS-containing serum on OGD insulted bEnd.3 cells barrier injury resulted from tPA treatment. H Knockdown of VIP in N2a cells attenuated the protective effects of SSS-containing serum on OGD insulted bEnd.3 cells barrier injury resulted from tPA treatment. 40 × images scale bar = 50 µm *p < 0.05 vs MCAo