Fig. 3

EA suppresses GABA^RVM neuronal activity in CFA- and CCI-treated mice. A Experimental design for recording GCaMP6s fluorescence signals from GABA^RVM neurons of CFA-treated mice. B Representative GCaMP6s fluorescence signals recorded from the Saline (left), CFA (middle), and CFA-EA (right) groups. C-E The area under the curve (AUC) (C), maximum ΔF/F (D), and mean peak amplitude (E) of GCaMP6s fluorescence signals were recorded from the Saline, CFA, and CFA-EA groups. F Experimental design for recording GCaMP6s fluorescence signals from GABA^RVM neurons of CCI-treated mice. G Representative GCaMP6s fluorescence signals were recorded from the Sham (left), CCI (middle), and CCI-EA (right) groups. H-J The area under the curve (AUC) (H), maximum ΔF/F (I), and mean peak amplitude (J) of GCaMP6s fluorescence signals recorded from the Sham, CCI, and CCI-EA groups. For C-E and H-J, significance levels are indicated as * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the CFA/CCI group, using one-way ANOVA with Dunnett’s post hoc test, n = 5 mice per group. K A schematic representation of electrophysiological recordings within the RVM in freely moving mice. The enlarged section highlights the multichannel tetrode. L The displayed are the raster plot (upper part) and the peri-stimulus time histogram (lower part) illustrating the multi-channel recordings of GABA^RVM neuronal firing rates in control mice before, during, and following von Frey (1.0 g) stimuli (n = 12 cells from 2 mice per group). M Overlay of contact-evoked (blue) and averaged spontaneous (navy Blue) spike waveforms from the example unit. N The firing rates of GABA^RVM neurons in control mice before, during, and after von Frey (1.0 g) stimuli, ** p < 0.01 and *** p < 0.001 vs. Contact group, repeated measures one-way ANOVA followed by Dunnett’s post hoc test, n = 12 cells from 3 mice per group. O Representative recording of spontaneous spikes and data P showing GABA^RVM neuronal firing rates in mice treated with saline, CFA, and CFA-EA (n = 15 cells from 3 mice for RVM::Saline; n = 12 cells from 3 mice for RVM::CFA; n = 14 cells from 3 mice for RVM::CFA-EA). Q Representative recording of spontaneous spikes and data R showing GABA^RVM neuronal firing rates in mice treated with Sham, CCI, and CCI-EA (n = 17 cells from 3 mice for RVM:: Sham; n = 15 cells from 3 mice for RVM:: CCI; n = 13 cells from 3 mice for RVM:: CCI-EA). Scale bars = 200 ms, ***p < 0.001 vs. CFA or CCI group, one-way ANOVA followed by Dunnett’s post hoc tests. All data are shown as mean ± SEM