Fig. 4

FVTF inhibits HCC cell stemness via targeting DNMT1/miR-34a-5p/FoxM1 axis. A–D DNMT1 overexpression (OE) antagonizes the effect of FVTF on formation ability of tumorsphere and soft agar colony (A), the percentage of CD133 positive cells (A), and the expression levels of CD44 protein (C) and BMI1, OCT4 and SOX2 mRNA (D) in the MHCC97H cells. E–H miR-34a-5p inhibitor antagonizes the effect of FVTF on formation ability of tumorsphere and soft agar colony (E), the percentage of CD133 positive cells (F), and the expression levels of CD44 protein (G) and BMI1, OCT4 and SOX2 mRNA (H) in the MHCC97H cells. I–L FoxM1 OE antagonizes the effect of FVTF on formation ability of tumorsphere and soft agar colony (I), the percentage of CD133 positive cells (J), and the expression levels of CD44 protein (K) and BMI1, OCT4 and SOX2 mRNA (L) in the MHCC97H cells. Scale bars = 100 μm. ^*P < 0.05; ^**P < 0.01; ^**P < 0.001; ^****P < 0.0001; ns, no significance