Fig. 6

AS-IV upregulates genes express associated with hematopoietic lineage and mitochondrial function. A Volcano plots depicting the DEGs in both the model and AS-IV treated groups. Red dots denote upregulated genes, while blue dots indicate downregulated genes (|log₂FC| > 1 and p-value < 0.05); B Hierarchical clustering analysis of AS-IV regulated DEGs; C Cellular radar map; D GO enrichment analysis of DEGs; E Enrichment analysis of DEGs in KEGG pathways. The positive or negative log₂FC value, the greater the fold change in gene expression; F–G Representative immunoblot images and biochemical quantification of mitochondrial energy metabolism-related pathway proteins (AMPK/PGC1α pathway) in bone marrow and spleen of different groups of mice after irradiation injury. Data represent the mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with model