Fig. 5

Glial cell-derived repair of TRPV1 neural axons to mediate EA to improve glycolipid metabolism. A, B Effect of EA on F4/80 (or EGF-like module-containing mucin-like hormone receptor-like 1), A, C Glial fibrillary acidic protein (GFAP) and A, D Glial cell-derived neurotrophic factor (GDNF) expression. Vinculin was used as an internal reference protein. E Representative IF images among groups. DAPI stained the nuclei (blue), the green immunofluorescence represents the GFAP and the red immunofluorescence represents the TRPV1. F, G Multiple EGF-like domains 10 (MEGF10) and F, H yeloid-epithelial-reproductive tyrosine kinase (MERTK) expression. Vinculin was used as an internal reference protein. I Typical IHC images of GDNF-positive labeling of the pancreas in various groups of rats. J Representative IF images and K quantitative fluorescence analysis of the pancreas in model group. The green immunofluorescence represents the TRPV1 and the red immunofluorescence represents the MERTK. ^#P < 0.05 vs. normal control, ^*P < 0.05 vs. model or another treatment group. TRPV1 transient receptor potential vanilloid 1, CA capsazepine, ST25 Tianshu acupoint