Fig. 3
From: Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
BA reduced lipid accumulation in LO2 and AML12 cells induced by alcohol. A Process diagram for modeling ALD and BA drug treatment in LO2 and AML12. B Western-blot detection of FASN protein expression in control, model group, and 6.25 μM BA, 12.5 μM BA, 25 μM BA drug group with 100 mM ethanol treatment. C Quantitative analysis of FASN. D Oil red O staining of LO2 slides in control, model group, and 6.25 μM BA, 12.5 μM BA, 25 μM BA drug group with 100 mM ethanol treatment, scale bar, 10 μm. E Quantitative analysis of oil red O staining in LO2. F–H qPCR detection of Fasn, Srebp1 and Apoa1 gene expression in AML12 cells from different groups. Data are expressed as the mean ± SD, n = 3–6 per group. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)