Fig. 4
From: Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
BA induced phenotypic polarization of RAW264.7 cells to M2-type cells in vitro. A RAW264.7 cells were exposed to control, 6.25 μM BA, 12.5 μM BA, 25 μM BA for stimulation respectively. B Protein immunoblotting of CD206, CD86, IL-2, CD163, CD206, and IL-4 expression levels in the control group and 6.25μM BA, 12.5μM BA, 25μM BA drug group. C–H Image J software analysis of CD80, CD86, IL-2, CD163, CD206, IL-4 expression levels vs. GAPDH or β-actin. I Immunofluorescence staining of CD206, CD86 and iNOS expression in the control group and 6.25 μM BA, 12.5 μM BA, 25 μM BA drug group. J–L Quantification of the mean fluorescence intensity of CD206, CD86 and iNOS using image J software. All data are shown as mean ± SD. n = 3–6 per group. Scale bar, 10 μm. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)