Fig. 6

Silencing Socs1 enhanced the polarization of RAW264.7 cells to the M1-type, whereas BA induces the repolarization of M1-type macrophages to the M2-type. The Socs1-siRNA RAW264.6 cells were constructed to block SOCS1 expression. Untreated RAW264.7 cells and Socs1-siRNA RAW264.7 cells were exposed to control, 25 μM BA and their conditioned medium for stimulation, respectively. A, B The effect of SOCS1 protein overexpression was analyzed by immunofluorescence staining and the quantification of SOCS1 in the control group and 6.25 μM BA, 12.5 μM BA, 25 μM BA drug group. C–E Western blot analysis of protein expression levels of CD163 and iNOS in the control group, 25 μM BA group, Socs1 siRNA group, and Socs1-siRNA + 25 μM BAgroup and quantification of expression levels of CD163 and iNOS vs. GAPDH using image J software. F–H Western blot analysis of protein expression levels of CD206 and CD86 in the control group, 25 μM BA group, Socs1 siRNA group, and Socs1-siRNA + 25 μM BAgroup and quantification of expression levels of CD206 and CD86 vs. β-actin using image J software. I, J The expression of IL-6 in the control group, 25 μM BA group, Socs1 siRNA group, and Socs1-siRNA + 25 μM BAgroup was analyzed by western blotting and quantified with image J software. K Immunofluorescence staining of CD206 and CD86 expression in the control group, 25 μM BA group, Socs1 siRNA group, and Socs1-siRNA + 25 μM BA group. L, M Quantify CD206 and CD86 expression in immunofluorescence staining using image J software. All data are shown as mean ± SD. n = 3–6 per group. Scale bar, 10 μm. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)