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Fig. 6 | Chinese Medicine

Fig. 6

From: Traditional Chinese medicine Youguiyin decoction ameliorate glucocorticoid-induced osteonecrosis in rat by modulating ROS/PHD2/HIF-1α oxidative stress signaling pathway in bone marrow mesenchymal stem cells

Fig. 6

Promoting effect of YGY medicated serum on osteogenic differentiation of dexamethasone-induced BMSCs. A Specific procedure and grouping of cell experiments. B Alizarin red staining results of each group of cells. Scale bar: 200 μm. C ROS fluorescence results for each group of cells. Scale bar: 100 μm. D Levels of oxidative stress for each group of cells (n = 6 technical replicates). E Number of calcium nodule deposits for each group of cells (n = 9 technical replicates). F Western blot results for each group of cells. G Quantitative analysis results of Western Blot (n = 3 independent experiments). Statistical analysis: Unpaired t-test. nsP > 0.05,*P < 0.05, **P < 0.01 and ***P < 0.001

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Chinese Medicine

ISSN: 1749-8546

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